Short Communication THE EVALUATION OF CHROMAGAR-CANDIDA AS AN ISOLATION MEDIUM FOR IDENTIFICATION OF CANDIDA SPECIES ISOLATED FROM DIFFERENT CLINICAL SPECIMENS
نویسنده
چکیده
The aim of the study was to evaluate the accuracy of CHROMagar-Candida for the identification of Candida spp. from different clinical specimens. The growth of cultures on plates, containing Sabouraud dextrose agar and CHROMagar-Candida were compared. Non-Candida albicans yeast isolates were further specificated with API 20C (bioMerieux, France). Of the 178 cultures processed, 100% accuracy for C. albicans C. krusei, C. tropicalis and 99% for other Candida spp. were determined. CHROMagarCandida was found to be a satisfactory isolation medium for different clinical specimens, allowing immediate and correct identification of the commonly encountered yeasts. The use of CHROMagar as a yeast isolation medium in a clinical laboratory for the routine examination appears to be extremely successful in primary isolation and differentiation medium in different sample collections (vaginal specimens, blood cultures, gaita, urine sample, sputum and oropharyngeal swabs) of yeast also it is more rapidly than classical methods.
منابع مشابه
Isolation and Identification of Candida Species from the Oral Cavity Using CHROMagar Candida
CHROMagar Candida is a new chromogenic culture medium that is used for the isolation and direct identification of some of the most clinically important yeast pathogens on the basis of colony colour. The aim of the study was to isolate and identify Candida species from the oral cavity with CHROMagar Candida. Samples (158) from 68 orthodontic patients wearing upper removable orthodontic applianc...
متن کاملEvaluation of CHROMagar Candida for rapid screening of clinical specimens for Candida species.
CHROMagar Candida is a new differential culture medium that allows selective isolation of yeasts and simultaneously identifies colonies of Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei. We evaluated this medium and compared it with a reference medium, Sabouraud glucose agar, for the presumptive identification of yeast species isolated directly on the medium from 1150...
متن کاملEvaluation of chromogenic media and seminested PCR in the identification of Candida species
Identification of Candida cultured from various clinical specimens to the species level is increasingly necessary for clinical laboratories. Although sn PCR identifies the species within hours but its cost-effectiveness is to be considered. So there is always a need for media which help in the isolation and identification at the species level. The study aimed to evaluate the performance of diff...
متن کاملIdentification of Candida Species Isolated from Hospitalized Patients with Candiduria
Background and objectives: The incidence of candiduria caused by Candida spp. has increased in recent years, particularly in hospitalized patients. Candiduria is most commonly caused by Candida albicans; however, an increase in the prevalence of non-albicans species has been observed during last decades. This study aimed at molecular identification of Candida species isolated from candiduria in...
متن کاملشناسایی گونههای کاندیدا با روشهای قارچشناسی و مولکولی در زنان باردار مبتلا به کاندیدیازیس واژینال
Background: Vaginal candidiasis is common in during pregnancy. It may lead to complications like abortions, premature birth, low birth weight, chorioamnionitis and fungal systemic neonatal infection. The aim of present study was identification of Candida species by mycological and molecular methods in pregnant women with vaginal candidiasis. Methods: This cross-sectional study was performed on...
متن کامل